8-Color STR Detection on the Spectrum Compact CE System
Poster
Karthika Divakaran1, Justin J. Lemke1, Heather L. Hodges1, Asami Terakado2, Yoshihiko Hagiwara2, Hodaka Kozakai2, Toshiaki Yanokura2, Isao Haraura2, Noriyuki Sumida2 and Robert S. McLaren1 1 Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711 2 Hitachi High-Tech Corporation, 882 Ichige, Hitachinaka-shi, Ibaraki, 312- 8504 Japan
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Promega was pleased to present a poster at this year’s International Symposium on Human Identification which showcased the 8-color STR detection capabilities of the Spectrum Compact CE System. Launched in 2020, the Spectrum Compact CE System, a four-capillary instrument, is capable of fragment analysis and sequencing applications. It features easy to install consumables, an intuitive touchscreen interface, Remote Access software, and recent increases in the Capillary Cartridge and Polymer Cartridge capacities. New later this fall, a free of charge software upgrade will enable the Spectrum Compact CE System to detect up to 8 dyes, allowing it to make use of the all current and future PowerPlex® 8-dye multiplexes.
The poster demonstrated the ability to detect 8 dyes simultaneously which allows for more DNA markers with smaller amplicons.
Left: PowerPlex® 35GY profile of 1ng 2800M Control DNA amplified for 29 cycles in 25µL reactions. Electrophoresis performed on the Spectrum Compact CE System. Data analyzed using GeneMarker® HID Software for Spectrum CE Systems.
Right: PowerPlex® 18E profile of 0.5ng 2800M Control DNA amplified for 30 cycles in 25µL reactions. Electrophoresis performed on the Spectrum Compact CE System. Data analyzed using GeneMarker® HID Software for Spectrum CE Systems.
The poster data established high sensitivity and robust results as well as good intra- and inter-dye balance and low bleedthrough for both the PowerPlex® 18E and 35GY Systems.
The Spectrum Compact CE System was tested for sensitivity against serially-diluted DNA samples. Both 8-color kits produced 100% profiles from their suggested inputs down to 62.5pg of DNA. At 31.25pg, the 35GY kit produced >95% profiles and the 18E kit produced 100% profiles.