ProNect® Data Platform

Product Updates

Updating MyGlo® Firmware

Instructions for updating your MyGlo firmware.

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Release Notes

Release Date: 07/17/2026

MyGlo firmware version: 57

Connect2ProNect version: 26.7.16.2

GloFi version: 6.6.52 NEW!

IMPORTANT: Update Connect2ProNect first!

Before updating your MyGlo firmware, make sure you are running Connect2ProNect version 26.7.16.2 or later. Updating firmware from an older version will leave your instrument in a state where the Read Plate Tool and new connection methods will not function — even though the firmware appears to have updated.

Do this in order:

  1. Download and install Connect2ProNect 26.7.16.2 using the button below.
  2. Open Connect2ProNect and update your firmware when prompted.

Already updated your firmware from an older version of Connect2ProNect? Install version 26.7.16.2 using the button below and re-run the firmware update. This will restore the Read Plate Tool and connection methods.

Instruments needing the update are tagged "Firmware update needed."

▼ Download Connect2ProNect 26.7.16.2

ProNect Platform:

Refreshed login experience:

  • A fresh look for the login screen with a new background image and updated Promega branding. All login functionality remains the same.

Dashboard & navigation:

  • Refreshed dashboard layout with a full-width Applications tile for a cleaner, more focused workspace.
  • Directly access MyGlo specific Application Notes and the new Pro Science Club from the Resource Center.

Looking for a dedicated app? Check the Coming Soon card on your dashboard to see which app workflows we have in development.

Instrument monitoring:

  • Instrument telemetry now updates at approximately one-minute resolution instead of being smoothed into large averages, so brief temperature and humidity spikes are visible.
  • The Instrument Dashboard “About” tab now shows the associated GloFi Serial Number, Config Method, and Last Config Date/Time when a GloFi was connected.
  • Consistent instrument status and remaining-time display across the dashboard, instrument dashboard, and instrument-selection screens.

My Data section enhancements:

  • Rename instrument data files and plate maps in place — no more “save a copy” workaround.
  • Click a file name to reopen its results (heat map view, or the Time Course screen for time-course files).
  • Read type now appears in its own column, and the downloaded file type is now Excel (not CSV).

Connect2ProNect (C2P): New version with expanded capabilities and functional updates

  • Redesigned, guided setup activates your device on ProNect, then walks you through connectivity setup step by step.
  • Supports 7 different methods (6 new) for connecting your MyGlo so you can find the best option for your situation.

New Connection Methods: (also see new MyGlo Setup Guide)

    • 5 GHz Wi-Fi (requires GloFi) — For a single MyGlo when your organizations network requires 5 GHz, or when 2.4 GHz has too much interference.
    • Ethernet (requires GloFi) — For a single MyGlo in labs with poor or unreliable Wi-Fi signal strength.
    • Wi-Fi Hotspot (requires GloFi) — GloFi connects to Ethernet and broadcasts a hotspot, supporting wireless connectivity for multiple MyGlos.
    • Cellular (requires GloFi) — For a single MyGlo in field or remote locations without network infrastructure; use your own SIM card. No IT support needed.
    • Cellular Hotspot (requires GloFi) — A cellular connection shared across multiple MyGlos for field or remote locations. No IT support needed.
    • Shared Connection (does not require GloFi) — For secure networks such as WPA3-Enterprise and eduroam, using a networked computer. Supports a single MyGlo.

Note: Five of these methods use the new GloFi™ Network Connectivity Adapter. 2.4 GHz Wi-Fi remains available as before.

  • Offline firmware updates: the latest MyGlo firmware is bundled with the installer, and diagnostic logs can be pulled from MyGlo and GloFi together.
  • Windows Service Relay (WSR) is included to enable as an optional component and is auto-detected when active.
  • Fixes:
    • Resolved an issue where activating with a 32-character device name on 2.4 GHz Wi-Fi could leave a device unrecoverable.
    • The device name field now correctly enforces 3–32 characters.
    • Safely close the app mid-update or activation and it resumes automatically.
    • The 'on-battery' warning is now a dismissible, non-blocking advisory.

GloFi™ Network Connectivity Adapter: NEW!

  • Connect multiple MyGlo readers to ProNect through a single GloFi acting as a wireless access point.
  • Automatically configures your connection — Ethernet, Wi-Fi, or Cellular — based on your MyGlo/Connect2ProNect setup (one connection type can be active at a time).
  • Use your own SIM card for cellular connections, with optional custom carrier network settings for plans that require them.
  • At-a-glance status LEDs communicate device state through distinct light patterns; the right LED pulses amber when no MyGlo is connected.
  • Built on a hardened, security-first operating system with locked-down access and stricter network rules to help reduce the risk of unauthorized access.

Windows Service Relay (WSR):

  • Used with Shared Connection method, installs with new Connect2ProNect software.
  • System tray icon shows live status (Offline, Idle, Ready, Busy, Error).
  • Convenient right-click options to start or stop the service, view logs, or exit the application.

MyGlo Read Plate Tool: NEW!

Efficiently conduct endpoint or time course reads (NEW!) for any Promega luminescence assay without using a dedicated app.

  • Configure wells and protocol, pick an instrument, confirm your plate, and read. Files auto-name with a timestamp and can be renamed before you start your read.
  • Select multiple wells quickly with click-and-drag.
  • Opt in to receive email and/or SMS notifications when a read completes (Settings > Notifications). For time-course reads, choose to be notified at completion, after every time point, or both. No notification is sent if a read fails or is canceled.
  • Completed reads display as a heat map with options to download results as an Excel file, open in a Cell Health app for analysis, or seamlessly start another read.
  • Time-course reads include a read-by-read results view, and a single Excel export bundles all completed reads together. Supports 2–72 reads (plus optional baseline), 15–60 minute intervals, 0.1–30 second integration time, and up to 72 hours total time course duration.
  • Remaining-time display now adapts its format automatically so it’s always easy to read at a glance.
  • Richer saved-file metadata (instrument name, serial number, integration time — plus reads count, interval, and baseline for time-course reads), with a clear flag for whether a time-course read finished or was canceled.
  • Read Plate Tool files (including time-course) now appear when selecting instrument data for analysis; time-course files are visible with an explanatory tooltip.

Cell Health App Enhancements:

CellTiter-Glo®, Caspase-Glo® 3/7, LDH-Glo™, and BacTiter-Glo™.

Visit the resource pages for each Cell Health app for “How To” videos and more.

  • Caspase-Glo 3/7 has been rebuilt around fold change and now supports Single Concentration, Dose Response, and Linearity analysis — each exportable to Excel (plate or sample format) and JSON, matching CellTiter-Glo. %CV columns are color-coded by replicate variability.
  • Reading screens updated to match the new Read Plate Tool layout and time-remaining format.
  • LDH-Glo now applies stronger plate map validation for Control, Background, and Sample/Max Release Control well grouping.
  • New plate maps no longer pre-assign well A1 as a Sample well.
  • “Edit Plate Map” is now “View Plate Map,” which opens a read-only snapshot from analysis time; “Save a Copy” creates an editable version.
  • LDH-Glo analysis: the Fold Dilution column is now hidden when using LDH (mUnit/ml) units, and the Cytotoxicity tab/export is hidden when no Max Release Control is present.
  • Numerous additional fixes across plate maps, graphs, tooltips, and data handling for a smoother analysis experience.
  • Read Plate Tool files (including time-course) now appear when selecting instrument data for analysis; time-course files are visible with an explanatory tooltip.

TPD App Enhancements:

Visit the TPD App Resource page for “How To” videos and more.

  • Plate map validation now requires at least two (2) zero-concentration sample wells per target and consistent replicate counts across non-zero concentrations.
  • A 20-character limit warning was added for the Compound, Target, and Cell Type fields.
  • The “Time Dmax Ends by Concentration” graph has moved to Degradation Metrics (data and calculations are unchanged).
  • Fixes for sample concentration display and for compound names containing underscores or dashes.

Join the Pro Science Club: NEW!

A global community built to move your science to the next level. Connect with peers, exchange bold ideas, and access exclusive content. Find details and join the conversation from the Resource Center in ProNect.

Release Date: 01/30/2026

MyGlo firmware version: 45 Connect2ProNect version: 25.5.25.1

ProNect Platform:

  • New instrument telemetry charts now display MyGlo and GloMax sensor data (temperature, humidity) over time to help assess environmental effects on experiments.
  • Users can now filter telemetry data by date range and export sensor data to Excel for offline analysis.
  • Improved instrument status messaging, including “Reading - HH:MM:SS” display during plate reads.
  • Redesigned app cards on the dashboard support direct "Read" actions to streamline workflow for frequent users.

BacTiter-GloTM App: NEW!

  • Measure ATP levels to assess viable microbial cells in a sample.
  • Visit the BacTiter-Glo App Resources page for "How To" videos and more.

Feature Enhancements:

New BacTiter-Glo™ App added to ProNect, supporting complete experimental workflows:

  • Design and save 96-well plate maps with support for Single Concentration, Dose Response, and Linearity Analysis.
  • Full compatibility with shared plate maps across Cell Health apps.
  • Read plates with or without a plate map using MyGlo, and generate heatmaps immediately.
  • Quantify sample values using a standard curve and export detailed data to Excel and PDF.
  • Analyze and visualize data through interactive graphs, including:
  • 4PL/5PL Dose Response curves with EC₅₀/IC₅₀ tables
  • Single Concentration bar charts
  • Linearity plots with regression equations
  • Standard Curve and Quantified Value overlays

Improved file management and access:

  • Access plate maps, instrument data, and results from “My Data” and Instrument Dashboard.
  • Rename and organize results directly within the app for improved file tracking.
  • Download a full analysis manifest, including raw data, plate maps, visualizations, and quantified results.

Analysis tools and visualizations:

  • Hover tooltips and toggles for error bars, units, and data series for in-depth data exploration.
  • PDF exports include all graphs and calculated metrics for offline reporting.

CellTiter-Glo App Enhancements: Visit the CellTiter-Glo App Resource page for "How To" videos and more.

CellTiter-Glo now supports Standard Curve-based quantification for Single Concentration and Dose Response workflows, with newly added visualizations for quantified values and a streamlined read-first workflow accessible directly from the app card.

  • Standard Curve support in CellTiter-Glo enables ATP-based quantification for Single Concentration and Dose Response workflows.
  • New visualizations for quantified values across analysis types now available in ProNect and PDF reports.
  • Read-first workflow option added to the Cell Health app cards for faster assay setup.

TPD App Enhancements: Visit the TPD App Resource page for "How To" videos and more.

  • Added in-app notification to recommend renormalization when initial fractional RLU is out of expected range.
  • New guidance modals during plate map setup help improve experimental accuracy.
  • Updated progress indicators clarify full workflow steps, including “Run Analysis”.

Release Date: 09/19/2025

MyGlo firmware version: 45 Connect2ProNect version: 25.5.25.1

ProNect Platform:

  • Launched a new Resource Center in the navigation panel for quick access to categorized support content and helpful links.
  • Introduced a Promotional Tile on the dashboard to surface key updates, new features, and announcements in real time.
  • Streamlined the Settings experience: account, password, and legal settings are now consolidated; notifications now include the ability to reset in-app Cell Health messages.
  • Users can now rename analysis result files after generation, with validation to prevent duplicate or invalid names.
  • Plate maps are now directly accessible from the My Data dashboard tile—no need to navigate to individual records.
  • Platform services upgraded to .NET 8 for enhanced performance and long-term compatibility.

Cell Health App Enhancements:

  • EC₅₀/IC₅₀ tooltip labels now include proper formatting and nomenclature for improved clarity during data review.
  • “Get a MyGlo” link now opens the MyGlo product page in a new browser tab to streamline device acquisition.
  • Start analysis and download plate read data directly from the heat map view.

TPD App:

  • Y- and X-axis values now appear correctly in exported PDFs for degradation metrics visualizations.
  • Added an in-app message during PDF generation to let users know downloads are in progress, improving transparency and reducing confusion when exporting multiple visualizations. Import template + instructions

Release Date: 07/18/2025

ProNect Platform:

  • All Instrument Data files now automatically appear in the My Data tile of your dashboard.
  • File pinning now available in My Data.
  • Quick link to MyGlo + ProNect Product Updates available in the Resource Center section.
  • Error messages clearly flag invalid characters in file names, helping avoid confusion during saving.

LDH-Glo™ App: NEW!

  • Analzye LDH release for cell viability and cytotoxicity workflows using your MyGlo.
  • Visit the LDH-Glo™ App Resources page for "How To" videos and more.

Cell Health App Enhancements:

App Workflow

  • Workflow updated to Read First, Full Plate read now default with less clicks to read. (see image below)
  • File pinning now available.
  • Start analysis and download plate read data directly from the heat map view.

Plate Maps

  • Background well type now optional.

Analysis Visualizations

  • EC₅₀/IC₅₀ summary tables available below dose-response charts.
  • Linearity graphs now anchor both x- and y-axes at (0,0).

Exported Analysis File

  • Instrument Data for complete app workflow, metadata includes well type, compound name, concentration, treatment time, and assay notes.

New App Resources

  • CellTiter-Glo® App Resources
  • Caspase-Glo® 3/7 Resources

TPD App:

Import TPD Kinetic Read Data

  • Import plate read files exported from a different brand of plate reader or a GloMax® instrument not connected to your ProNect account.
  • Import template + instructions

PDF Export for Analysis Visualizations

  • PDF export for Single Concentration and Dose Response visualizations enables you to export high quality graphs for easy reporting.

Release Date: 06/10/2025

System Enhancements:

  • Performed backend updates to improve overall system performance, scalability, and reliability. These updates are non-disruptive, require no user action, and do not affect validated workflows, data integrity, or user-facing functionality.

Release Date: 01/31/2025

Connect2ProNect version: 24.12.18.1

Wi-Fi & Setup Improvements

  • IMPORTANT: Download and install the latest version of Connect2ProNect to ensure your MyGlo firmware can be successfully updated.
  • Users now receive support information during Wi-Fi setup.
  • Prompt added to close the C2P app after activating MyGlo.
  • MyGlo reader now automatically appears on the ProNect dashboard after setup.

Deactivation Process Enhancements

  • Users can now choose to retain or delete reader data during deactivation.
  • Retained data remains accessible from deactivated instruments.
  • Users can download reader data during deactivation via MyGlo.

MyGlo firmware version: 41

  • Bug Fix: Resolved an issue where the instrument incorrectly triggered a false positive shutter error.
  • See instructions for updating your MyGlo firmware.

TPD App:

Plate Map & Concentration Controls

  • New option to select Single Concentration or Dose-Response as the analysis type.

View video "Using the Single Concentration Analysis"

  • Users can choose concentration units in molarity or mass/volume.
  • Plate map now limits displayed values to four decimal places for improved clarity.

Data Management & Analysis Enhancements

  • Users can now analyze aborted instrument data files.
  • Support for kinetic data file imports from GloMax instruments into the TPD app for analysis.

View video "Importing GloMax Data Files for Analysis"

  • Downloaded folders from the instrument dashboard now retain the original file name.

Resource Accessibility

  • Users can now access the app datasheet and tutorial video directly from the app.

Improved Data Visualization

  • Enhanced data visualizations now include:

Time DMax absolute Recovery rate by concentration Maximum recovery (Rmax) as % of initial and degraded protein Time Rmax absolute Time Dmax start & end Fitted curve of AUC (area under kinetic degradation curve)

  • Graph values now display up to four decimal places for consistency.

Release Date: 11/26/2024

CellTiter-Glo and Caspase-Glo 3/7 Apps:

  • Instrument data files (plate read results) will now download in Excel format to improve data portability.
  • Raw RLU data is now available in both tabular and plate map format in analysis output files.
  • Heatmap view will now appear after a Full Plate Read providing a quick visual data check.

View video of full plate read for CellTiter-Glo App

View video of full plate read for Caspase-Glo 3/7 App

Release Date: 09/11/2024

MyGlo firmware version: 40

  • Added the ability to start a plate read remotely from the app to improve flexibility in your workflow allowing you to insert a plate and then initiate the read.
  • Improved automatic plate detection performance.

Connect2ProNect version: 24.7.17.1

  • Automated the process for requesting an activation code for MyGlo, simplifying device onboarding if the original code is lost or during reactivation.
  • Added functionality to refresh Wi-Fi networks and view passwords.

ProNect Platform:

  • Enhanced dashboard functionality for easier access to apps, resources, and instruments.
  • Improved navigation and usability with better scaling across all screen sizes.
  • Implemented significant updates to ensure proper content scaling on various resolutions, from HD to 4K.
  • Added notifications for new firmware availability in both the User Dashboard and Instrument Homepage, providing clear indicators when updates are needed.
  • Included the ProNect Data Platform Security Whitepaper in Settings under the Legal section.

CellTiter-Glo App:

  • Expanded MyGlo’s analysis capabilities with the addition of Linearity Analysis, see video here.
  • Enabled dragging and applying concentration series in multiple directions.
  • Added functionality to download analysis visualizations in PDF format, with support for 4PL and 5PL curve fitting.
  • Introduced features for inspecting single series data, displaying power regression graphs, and supporting linear regression with ATP standards or cell dilution.
  • Added the ability to start a plate read from the user interface, see video here.
  • For full plate reads, added the option to immediately read another plate and rename the read file, see video here.
  • Introduced quick access to tutorial videos and app datasheets from the application homepage for easy reference while using the app.

Caspase-Glo 3/7 App: NEW!

  • New app for analyzing Caspase-Glo 3/7 assays using MyGlo.
  • To learn more view the app video tutorial here and app datasheet here.

Release Date: 06/17/2024

MyGlo firmware version: 29

Connect2ProNect version: 24.5.9.2

ProNect Platform:

  • Responsive design updates to enhance viewing experience across different display resolutions.

App Updates, CellTiter-Glo App:

  • Integration time updated to support fractional values from 0.1 to 30 seconds.
  • Fit coefficients added to EC50 value tooltips for easy reference during data analysis.
  • Standardized to four significant figures for x-axis, fit coefficients, and concentration values.
  • For Single Concentration graphs, added the ability to change x-axis units to expand functionality.

Updating Your MyGlo Firmware

1. Connect your MyGlo

Use the USB cable to connect your MyGlo to the computer with the Connect2ProNect software installed.

2. Open Connect2ProNect

Open the Connect2ProNect software and log in to your ProNect account.

3. Firmware updates automatically

Connect2ProNect will automatically update your MyGlo's firmware and guide you through any necessary steps.

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